篇名 | 臺灣豐和黑毛烏骨雞之微衛星遺傳標記多態性分析 |
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卷期 | 46增刊 |
並列篇名 | Genetic diversity analysis of TW Fengho black silkie chicken by microsatellite markers |
作者 | 林德育 、 曾淑貞 、 王義松 、 劉曉龍 、 賴永裕 、 吳明哲 |
頁次 | 153-153 |
關鍵字 | 黑毛烏骨雞 、 遺傳多樣性 、 微衞星遺傳標記 、 Black silkie chicken 、 Genetic diversity 、 Microsatellite |
出刊日期 | 201712 |
為評估臺灣豐和黑毛烏骨雞選育族群的遺傳變異,本試驗利用 FAO(2011)建議 使用的 24 組雞微衛星標記組分析 48 隻臺灣豐和黑毛烏骨雞第 G5 世代種雞個 體 DNA。24 組微衛星標記皆有多態型的基因型。共檢測到 89 個對偶基因,平 均每個基因座具有 3.7 個對偶基因(2~9 個),其期望異質度介於 0.010 到 0.828, 平均為 0.517,觀測異質度介於 0 到 0.688,平均為 0.408,而多態性訊息含量平 均為 0.452。在選用的 24 組微衛星標記組中有 10 組呈現高度多態性資訊(PIC ≥ 0.5),有 11 組呈現中度多態性資訊(0.5> PIC ≥ 0.25),3 組呈現低度多態性資訊 (PIC < 0.25)。本結果提供此選育族群遺傳變異之基本分子資訊。
In order to evaluate genetic variation of TW Fengho black silkie chicken flock. we use a set of 24 microsatellite markers recommended by FAO to analyze 48 candidate bred chickens from this flock. All the microsatellites were polymorphic. The average allelic number was 3.7, ranged from 2 to 9 per locus. The expected heterozygosity ranged from 0.010 to 0.828, and the average expected heterozygosity was was 0.517±0.187(mean±SD). The observed heterozygosity of the population ranged from 0 to 0.688, and the average observed heterozygosity was 0.408±0.213. The estimated average polymorphic information content (PIC) was 0.452±0.171. In 24 markers, ten markers were highly informative with polymorphism information content (PIC ≥0. 50), eleven markers were reasonably informative(0.5> PIC≥ 0.25) and the other three markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of the population.