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中國畜牧學會會誌

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篇名 豐輝下營紅牌土雞之微衛星遺傳標記多態性分析
卷期 46增刊
並列篇名 Genetic diversity analysis of Fenghui Xiaying Red-Brand country chicken by microsatellite markers
作者 林德育曾淑貞楊豐輝賴永裕林秀蓮吳明哲
頁次 154-154
關鍵字 遺傳多樣性微衞星遺傳標記ChickenGenetic diversityMicrosatellite mar
出刊日期 201712

中文摘要

為評估豐輝下營紅牌土雞選育族群的遺傳變異,本試驗利用 FAO(2011)建議使 用的 24 組雞微衛星標記組分析 48 隻豐輝下營紅牌土雞第 G5 世代種雞個體 DNA。24 組微衛星標記皆有多態型的基因型。共檢測到 90 個對偶基因,平均每 個基因座具有 3.8 個對偶基因(2 至 8 個),其期望異質度介於 0.118 到 0.822, 平均為 0.492±0.202,觀測異質度介於 0 到 0.688,平均為 0.349±0.195,而多態 性訊息含量平均為 0.434±0.194。在選用的 24 組微衛星標記組中有 6 組呈現高 度多態性資訊(PIC ≥ 0.5),有 11 組呈現中度多態性資訊(0.5> PIC ≥ 0.25),7 組 呈現低度多態性資訊(PIC < 0.25)。本結果提供此選育族群遺傳變異之基本分子資 訊。

英文摘要

In order to evaluate genetic variation of Fenghui Xiaying Red-Brand country chicken flock. we use a set of 24 microsatellite markers recommended by FAO to analyze 48 candidate bred chickens from this flock. All the microsatellites were polymorphic. The average allelic number was 3.8, ranged from 2 to 8 per locus. The expected heterozygosity ranged from 0.118 to 0.822, and the average expected heterozygosity was 0.49±0.20. The observed heterozygosity of the population ranged from 0 to 0.688, and the average observed heterozygosity was 0.349±0.195. The estimated average polymorphic information content (PIC) was 0.434±0.194. In 24 markers, six markers were highly informative with polymorphism information content (PIC ≥ 0.50), eleven markers were reasonably informative(0.5 > PIC ≥ 0.25) and the other seven markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of the population.

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