篇名 | 聖約翰草對3T3-L1 脂肪細胞功能與概日節律基因表現之影響 |
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卷期 | 46增刊 |
並列篇名 | The effects of Saint John’s wort on the functions and the expression of circadian-related genes in 3T3-L1 adipocytes |
作者 | 李儒芸 、 崔郁棻 、 陳珠亮 |
頁次 | 203-203 |
關鍵字 | 脂肪細胞 、 約日節律基因 、 脂質蓄積 、 聖約翰草 、 Adipocytes 、 Circadian genes 、 Fat accumulation 、 Saint John’s wort |
出刊日期 | 201712 |
為提高畜產動物之飼料效率與降低飼養成本,本研究探討聖約翰草萃取物(Saint John’s wort extract, SJWE)對脂肪細胞脂質蓄積功能與概日節律的影響。3T3-L1 脂肪細胞分 化過程中以 SJWE 處理,由 oil-Red O 脂質染色與三酸甘油酯含量分析,顯示 SJWE 可降低脂肪細胞的油脂堆積。根據相對定量聚合酶連鎖反應分析結果,SJWE 會降低 脂肪細胞分泌激素Adiponectin 與Leptin mRNA 之表現。此外,SJWE 可使同步化標 幟基因Per2 於處理後 1 小時提升,並於第 8 小時降至低點,使細胞生理時鐘同步 化;並可使 Per2、Bmal1、Rev-erbα mRNA 表現節律性變化較為鮮明。最後,細胞 Leptin 分泌量分析顯示,SJWE 可顯著降低 Leptin 分泌量,而此抑制能力會隨時間 而有週期性變化。綜合上述結果,SJWE 可抑制 3T3-L1 脂肪細胞脂質蓄積與細胞激 素基因之表現,並調節約日節律。這些作用使 SJWE 具潛力用於改善畜產動物之飼 料效率。
In order to improve obesity and metabolic disorders in human and feed conversion in domestic animals, this study were performed to investigate the effects of Saint John’s wort extracts (SJWE) on fat accumulation and biological clock of adipocytes using 3T3-L1 adipocyte model. Fat accumulation of adipocytes was decreased by SJWE treatments as evidenced by morphological observation, oil-Red O staining and triglycerides assays. The mRNA levels, measured by RT-PCR analysis, of Adiponectin and Leptin, were lower in the SJWE-treated cells than those in the control cells. In addition, SJWE synchronized the biological clocks of adipocytes, as evidenced by the mRNA analysis of circadian- and metabolism- related genes. Taken together, SJWE has effects on regulating lipid metabolism, adipokine secretion, and biological clocks of adipocytes, and may be used for the improvement of metabolic disorders in human and feeding efficiency in domestic animals.