篇名 | 異地保種小型豬族群基因多樣性分析 |
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卷期 | 46增刊 |
並列篇名 | Genetic diversity of miniature pig population under ex-situ conservation |
作者 | 廖仁寶 、 陳若菁 、 陳佳萱 、 吳昇陽 、 許晉賓 、 陳立人 、 吳明哲 |
頁次 | 172-172 |
關鍵字 | 異地保種 、 基因多樣性 、 小型豬 、 Ex-situ conservation 、 Genetic diversity 、 Miniature pig |
出刊日期 | 201712 |
本研究目的在建立異地保種小型豬族群之遺傳背景資料。將 19 頭豬粒線體 DNA 部分 D 環區片段之 PCR 產物進行定序,可得長度為 616 bp 之 DNA 片段序列, 序列進行比對後,發現所有序列皆相同,再將此序列以比對工具 BLASTN 與 NCBI GenBank 進行比對,結果最為相近的序列為先前上傳登錄至 GenBank 的蘭 嶼豬粒線體 DNA 序列。以 12 種微衛星遺傳標記分析 19 頭蘭嶼豬 DNA,基因 多樣性參數平均交替基因數、觀測異質度、期望異質度及多態性訊息量數值分別 為 4.5、0.52、0.57 及 0.51,由多態性訊息量數值可得知族群的基因多樣性程度 屬高度(> 0.5),但仍需採用計畫性配種制度方能維持族群之基因多樣性。此外, 在 12 種標記的分析中,可發現標記 SW2008 僅有一種交替基因,未來可當作此 族群後裔的驗證標記。
The study was to establish the genetic background data of the ex-situ miniature pig population. The PCR products of the partial mtDNA D-loop from 19 pigs were sequenced to obtain a 616-bp fragment. After the DNA sequences were aligned, all the sequences were found to be identical. The closely related sequences found in the NCBI GenBank were those Lanyu pigs previously registered before by BLASTN analysis. The genetic parameters of average number of alleles, observed heterozygosity, expected heterozygosity and polymorphism information content of 19 pigs were analyzed by 12 microsatellite markers. The values of the genetic parameters were 4.5, 0.52, 0.57, and 0.51, respectively. The value of polymorphism information content showed that the genetic diversity of the population is high (> 0.5). However, it still needs to adopt the planned breeding system in order to maintain the genetic diversity of ex-situ groups. In addition, it was found that marker SW2008 had only one allele and could be used as a marker for the descent certification in the future.