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台灣農業研究

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篇名 青枯病菌(Ralstonia solanacearum Race 1/Biovar 1/Phylotype II/Sequevar 7)引起之番茄青枯病
卷期 69:4
並列篇名 Occurrence of Ralstonia solanacearum Race 1/Biovar 1/Phylotype II/Sequevar 7 Causing Tomato Bacterial Wilt in Taiwan
作者 蔡佳欣黃淑苓李佳蓉林靜宜呂昀陞
頁次 274-285
關鍵字 青枯病菌第1生理小種第1生化型第2演化型第7序列變種Ralstonia solanacearumRace 1Biovar 1Phylotype IISequevar 7
出刊日期 202012
DOI 10.6156/JTAR.202012_69(4).0002

中文摘要

宜蘭縣地區一處番茄栽植田於2016–2017年出現番茄植株生長不良及萎凋現象,萎凋之番茄植株枝葉仍呈青綠色,與青枯病徵狀相似。將罹病番茄莖部切開,可見維管束組織褐化蔓延,從病組織可在triphenyl tetrazolium chloride培養基分離出與青枯病菌相似的中間粉紅色,周圍白色流質狀之細菌菌落。該細菌分離株經生理生化特性分析、Biolog細菌鑑定系統測試、16S rDNA序列比對、青枯病菌專一性引子對,將該分離株鑑定為Ralstonia solanacearum。進一步再經寄主範圍測試、生化型測試、青枯病菌演化型鑑別之引子對及親緣關係分析,將其歸類為第1生理小種(race 1)/第1生化型(biovar 1)/第2演化型(phylotype II)/第7序列變種(sequevar 7)。又將該分離株進行科霍氏法則試驗,接種番茄植株後可造成與田間所見相同之萎凋病徵,並從罹病植物再分離出相同的第2演化型青枯病菌。該分離株尚無在台灣危害番茄的紀錄,此為青枯病菌第1生理小種/第1生化型/第2演化型/第7序列變種感染番茄在台灣的首度報導。

英文摘要

During 2016–2017, growth decline and wilt were observed in a number of tomato plants in a tomato planting field in Yilan County. The leaves of the wilt tomato plants remained greenish, similar to the symptoms of the bacterial wilt. The stems of the diseased tomato were cut open, and the vascular tissues were found browning in appearance. The bacteria colonies isolated from the diseased stems were fluidal, white with pink center on triphenyl tetrazolium chloride medium. Based on physiological and biochemical properties, Biolog identification system, 16S rDNA sequence analysis and specific primer pair, the bacteria were identified as Ralstonia solanacearum. The bacteria were further classified as race 1/biovar 1/phylotype II/sequevar 7 by the host range, biovar test, phylotype multiplex polymerase chain reaction and phylogenetic analysis. In Koch’s postulates, the inoculated tomato plants developed wilt symptoms as those observed in the field, and the same bacteria were re-isolated from symptomatic plants. This is the first report of R. solanacearum race 1/biovar 1/phylotype II/sequevar 7 causing tomato bacterial wilt in Taiwan.

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