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中華民國泌尿科醫學會雜誌

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篇名 Identification of Proteus Mirabilis Urease Gene and 16S Ribosomal DNA in Urinary Calculi by Polymerase Chain Reaction
卷期 7:2
並列篇名 以聚合酶鏈反應偵測尿石中的變形桿菌尿素酵素基因及16S核糖去氧核糖核酸
作者 王中敬陳淳許德金賴明坤
頁次 65-70
關鍵字 尿石磷酸銨鎂尿石變形桿菌尿素酸素聚合酶鏈反應urinary calculistruviteProteus mirabilisureasepolymerase chain reactionTSCI
出刊日期 199606

中文摘要

利用聚合酶鏈反應偵測95個磷酸銨鎂(或含磷酸鈣)尿石,以確定是否含變形桿菌尿素基因及16S核糖去氧核糖核酸。聚合酶鏈反應的結果顯示在95個受檢驗的尿石中,31個存有變形桿菌尿素酸素基因,58個含16S核糖去氧核糖核酸。於陽性聚合酶鏈反應的尿石中,22個尿石的尿液培養同時含變形桿菌,此外2個陰性尿液養結果的尿石也呈陽性聚合酶鏈反應。我們的結論認為以聚合酶鏈反應來檢測尿石內變形桿菌細胞片段的存在與否是可行,而且非常方便。

英文摘要

Ninety-five urinary tract stones, composed of struvite with/without apatite, were examined by polymerase chain reaction( PCR )for the presence of Proteus mirabilis urease gene and eubactertal 16S ribosomal DNA. PCR demonstrated the presence of urease gene in 31, and 16S ribosomal DNA in 58, of the 95 calculi investigated. PCR was not only positive in 22 calculi in which Proteus mirabilis was discovered by urine culture, but also in 2 cases with negative urine culture. It was concluded that the PCR method is feasible and convenient for identifying the existence of Proteus mirabilis as viable or nonviable cells within urinary calculi.

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