Interface quality, customer perceived security, switching costs, customer perceive usability, customer satisfaction and customer loyalty are critical factors for the success of an e-commerce website. Which one is the most important factor and which one is the least important? E-commerce companies need to improve themselves by keeping improve the website, technology and organization well. In this study, data collected from the survey of 104 respondents that was tested by a designed conceptual model and its researching hypotheses. The analytical results demonstrated that interface quality, perceived security, switching cost, perceived usability positively all affected customer satisfaction and customer loyalty on the e-commerce. Overall, all four factors have significance as important factors on customer satisfaction and customer loyalty. However, about the difference of significances of four regressions from these four factors, perceived usability and interface quality could be the most important factors to build customer satisfaction on E-commerce website with the highest score. The least important factor was the perceived security. Finally, the findings in this study expected to provide helpful guidelines to the management of e-commerce.