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中西整合醫學雜誌

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篇名 柚皮素對HBx 誘導肝癌細胞轉移之影響
卷期 21:1
並列篇名 Effect of HBx-induced Metastatic Microenvironment by Naringenin in Hepatocellular Carcinoma
作者 鍾郁蓉葉家舟
頁次 008-020
關鍵字 柚皮素HBx基質金屬蛋白酶9血管內皮生長因子腫瘤侵襲NaringeninHBxMatrix metalloproteinase 9VEGFTumor invasion
出刊日期 201906

中文摘要

柚皮素是一種大量存在於水果、蔬菜和中草藥中的黃酮類化合物,具有很強的抗增殖特性和誘導肝癌細胞凋亡的能力。然而,尚未研究柚皮素對預防HBx 誘導的肝癌細胞侵襲的作用。方法:為了確定HBx 是否調節遷移,侵襲和生長因子的表達,我們女,將檢驗HBx和HBx-GFP 融合蛋白是否在用或不用DOX 處理的HBx 轉染細胞中表達。結果: 柚皮素通過抑制激活蛋白(AP)-1 和核因子-κB(NF-κB) 活性抑制MMP-9 轉錄, 並通過抑制細胞外磷酸化抑制TPA 誘導的AP-1 活性。信號相關激酶(ERK)和c-Jun N 末端激酶(JNK)信號通路。柚皮素還通過IκB 抑制HBx 誘導的NF-κB 核轉位抑制。結論:這些數據表明,柚皮素通過抑制多種信號傳遞導途徑抑制肝細胞癌的侵襲性和轉移潛能。因此,柚皮素可具有抑制肝細胞癌侵襲和轉移的治療潛力,並且可作為全身治療的潛在用藥。

英文摘要

Background:Naringenin, a flavonoid abundantly presents in fruits, vegetables, and in Chinese herbs, possesses strong anti-proliferative properties and the ability to induce apoptosis on hepatoma cell lines. However, the effects of Naringenin on preventing HBxinduced invasion of hepatoma cells have not been studied. Methods:To determine whether HBx modulates migration, invasion, and the expression of growth factors, we examined whether HBx and the HBx-GFP fusion protein were expressed in HBx-transfected cells treated with or without DOX. Results:Naringenin suppressed MMP-9 transcription by inhibiting activator protein (AP)- 1 and nuclear factor-κ B (NF-κB) activity and suppressed TPA-induced AP-1 activity through inhibition of phosphorylation in the extracellular signal-related kinase (ERK) and c-Jun N-terminal kinase (JNK) signaling pathways. Naringenin also suppressed Hbxinduced inhibition of NF-κB nuclear translocation through IκB. Conclusions:These data suggest that naringenin suppresses the invasiveness and metastatic potential of hepatocellular carcinoma cells by inhibiting multiple signal transduction pathways. Naringenin thus may have the therapeutic potential to inhibit the invasion and metastasis of hepatocellular carcinoma and act as a potential drug for systemic therapies.

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