文章詳目資料

Annals of Nuclear Medicine and Molecular Imaging

  • 加入收藏
  • 下載文章
篇名 Hook Effect of IRMA within the Range of Calibrators
卷期 23:3
並列篇名 IRMA校正濃度範圍內之Hook Effect
作者 曾翠芬鍾相彬張淑敏陳毓雯
頁次 137-141
關鍵字 胎兒蛋白變異係數hook-effectAFPCA125IRMAcoefficient of variation
出刊日期 201009

中文摘要

背景:高劑量hookeffect為一種使抗原抗體結合之免疫反 應檢項得到偽低值的現象,文獻報導受影響的檢項包括CA125、CA199、prolactin等。本研究探討在校正濃度範 圍內胎兒蛋白(alpha-fetoprotein;AFP)和CA125二檢項是 否有偽低值的報告出現。
方法:從2008年3月至2009年1月挑出利用immunoradiometric assay (IRMA)法檢測AFP結果介於500-780 ng/ml以 及CA125結果介於350-500U/ml的檢體,利用套裝試劑所提供的稀釋液作1:10稀釋或連續稀釋到報告出來之倍 數,再進行分析,並與原數據比較。
結果:“筆AFP檢測值介於500-780ng/ml ' 34筆CA125 檢測值介於350-500U/ml0稀釋後AFP有3個報告是原來 的5倍以上、1個報告是原來的4.88倍;CA125有3個報告 是原來的3.38倍以上。此二檢項各有一筆首次檢驗結果大於最高校正濃度,但再次檢驗結果卻是在校正濃度內,稀釋後的檢驗結采與原報告中目I司。AFP和CA125檢項稀釋以後得到的範固與原來的報告已有差距;AFP檢 項稀釋後有39.39毛的數據其變異係數(coefficientof variance; CV)大於159毛, CA12日合項稀釋後有35.39毛的結果 CV大於159毛。將此二檢項所選定未稀釋前的範圍分為三 個區段,AFP分成(1) 500-599.9 ng/ml、(2) 600-699.9 ng/ml和(3) 700-780 ng/ml ' CA125分成(1) 350-399.9 U/ml、(2)400-449.9 U/ml和(3)450-500 U/ml0其稀釋 前、後的結果變異係數大於159毛者以單因子變異數分析
結論:本研究發現hookeffect確實存在於少數病人的檢查 報告。就選定的範圍內沒有明顯的結采可以確認hookeffect會出現的濃度,但檢測結果若落在校正範圍內較高 區域時hookeffect應予以重況。

英文摘要

Background: High-dose hook effect is a phenomenon associated with an immunometric assay. It may give falsely low results for several tumor markers, such as CA125, CA199, prolactin, etc. The aim of this study was to investigate if falsely low value report appears in alpha-fetoprotein and CA125 analyses within the range of calibrators.
Methods: From March 2008 to January 2009, the samples of immunoradiometric assay (IRMA) for AFP with data of 500-780 ng/ml and for CA125 with data of 350-500 U/ml were collected. The samples were subject to 1:10 dilution or more with assay kit dilution buffer prior to analysis. The data of diluted samples were compared with undiluted ones.
Results: Sixty-one AFP and 34 CA125 samples were rechecked. After dilution, 3 AFP data were 5 times higher than the original data, and 1 specimen was 4.88 times higher. Three CA125 data were ³3.38 times than the original data. There were 39.3% and 35.3% of diluted AFP and CA125 specimen, respectively, with coefficient of variation (CV) ³15%. Among the data groups before dilution (AFP: 500-599.9 ng/ml, 600-699.9 ng/ml and 700-780 ng/ml; CA125: 350-399.9 U/ml, 400-449.9 U/ml and 450-500 U/ml), we statistically rechecked the ones showing CV ³15%, and an insignificant difference (P >0.05) was observed.
Conclusion: Hook effect was founded in some reports of immunometric assay. There was no solid evidence in selected data range to identify which concentration may induce hook effect. Nevertheless, we should carefully recheck the high concentration data in order to reduce the incidence of hook effect.

相關文獻