小果番茄為臺灣重要之經濟作物，常以嫁接於茄砧的方式栽培，具有抗土傳病害、耐淹水及耐旱等特性。目前臺灣小果番茄嫁接苗多仰賴人工嫁接，未來恐面臨農業人口老化或缺工等問題，有賴發展自動化嫁接模式。本試驗以不同光質進行茄砧‘EG203’ 與小果番茄接穗‘Rosada’ 育苗，調查苗株的形態特性、相對葉綠素含量及葉綠素螢光參數等生理特性；並進一步利用EMP-300 型嫁接機、嫁接輔助機及人工嫁接，進行嫁接測試，找出最適合嫁接機使用之茄砧及接穗的育苗環境。光質處理分為T5 燈管之全光譜白光、T5 燈管加紅光LED 燈、T5 燈管加藍光LED 燈、T5 燈管加遠紅外光LED 燈等4 種，並以溫室栽培者為對照組；試驗結果顯示，對照組及T5 燈管加遠紅外光LED 燈處理之苗株，不適用於嫁接苗操作。利用T5 燈管之全光譜白光、T5 燈管加紅光LED 燈、T5 燈管加藍光LED 燈處理之苗株，其莖徑(2.7、2.4、2.4 cm) 及下胚軸長度(4.3、4.7、4.3 cm) 適中，有較佳的生長勢，且機械嫁接速率高(13.1 ～ 17.9、11.8 ～ 18.2、12.1 ～ 17.6 sec plant-1)，有利於嫁接自動化的推行，為較符合嫁接機苗株規格之育苗環境。現有技術： 臺灣地區所栽種之小果番茄多以嫁接苗栽培，目前多以人工嫁接為主，未來恐面臨人口老化及季節性缺工等問題。創新內容： 本試驗擬以不同光質處理，並以不同嫁接方式進行比較試驗，期能找出最適嫁接機使用之茄砧及接穗的育苗環境。對產業影響： 可提供業界參考不同光質處理之育苗模式，並比較不同嫁接方式，有助於嫁接自動化的發展，以舒緩人口老化及季節性缺工等問題。

Cherry tomato is an important economic crop in Taiwan. It is often grafted onto eggplant to resist soil-borne disease bacterial wilt and abiotic stress such as water stresses. In the vegetable seedling grafting industry, manual grafting is most commonly used. The aging of farmers resulted in shortage of labors. In order to solve the problem, grafting machines were developed to replace hand-labor. In this research, we prepared tomato and eggplant seedlings under different light quality, including T5 fluorescent lamp, T5 plus red LED, T5 plus blue LED, T5 plus far-red LED, and greenhouse raised seedlings were used as control. The seedlings morphological characteristics, relative chlorophyll content, and chlorophyll fluorescence were investigated for each treatments. Three grafting method including by EMP-300 grafting machine, grafting auxiliary equipment and manual grafting, were used to graft tomato ‘Rosada’ scions onto eggplant ‘EG203’ rootstocks and the grafting efficiency among three methods was evaluated. The results showed that treatments of T5 fluorescent lamp, T5 plus red LED, and T5 plus blue LED, produced seedling with qualified stem diameter (2.7, 2.4, 2.4 cm) and hypocotyl length (4.3, 4.7, 4.3 cm), respectively, suitable for grafting machine. Nursing tomato and eggplant seedlings under those light quality can obtain not only vigorous seedlings but also good grafting efficiency (13.1-17.9, 11.8-18.2, 12.1-17.6 sec plant-1) and was proved to be the better nursery condition for machinery grafting. What is already known on this subject? In the vegetable seedling grafting industry, manual grafting is most commonly used. What are the new findings? We planted tomato and eggplant seedlings under different light quality conditions and expected to find a suitable condition to nurse tomato and eggplant seedlings for grafting machines. What is the expected impact on this field? It can be used for the farmers as a reference to select different light quality nursery condition. Hope to improve development of automatic grafting to solve shortage of labors problem.