Objective: This study aimed to explore the factors related to the knowledge and attitude of the intensive care unit (ICU) medical team towards palliative care. Methods: A cross-sectional study was conducted among members of the ICU medical team who had worked for more than six months in a hospital in northern Taiwan. A total of 220 valid questionnaires were completed through structured questionnaire surveys, including background information, palliative care knowledge scale, palliative care attitude scale, one-way ANOVA, Pearson correlation coefficient and regression analysis. Results: The average score of intensive care unit medical staff in palliative care knowledge was 18.95 (SD=1.81) points, with a correct rate of 86.14%; The average score of intensive care unit medical staff in palliative care attitude was 60.28 (SD=6.71) points. Among them, the statistical quantity of intensive care unit medical staff in demographic attributes, job attributes and education experience reached a significant level (p <0.05). Using Pearson correlation analysis method for statistical analysis, there was no significant correlation between the “palliative care knowledge scale” and “palliative care attitude scale” (r =-0.02, p =0.78). The predictive factors for medical staff's knowledge and attitude towards palliative care were tested by multiple regression model F test at a significant level (F=3.2, p <0.001). The t-test results showed that higher knowledge of medical staff in palliative care tended to provide end-of-life patients with palliative medical care. Conclusion: The more comprehensive the knowledge of end-of-life palliative care among ICU medical team members, the better the care they can provide for critically ill patients. It is recommended to plan palliative care training and education activities, encourage participation in palliative care seed training, obtain various palliative care certifications, improve the basic concepts and practical learning of palliative care among various healthcare professionals, and through communication and cooperation within the medical team, improve the quality of end-of-life care for patients, and achieve the goal of a peaceful death and no regrets for their families.