防己為常用中藥，是Aristolochiaceae或Menispermaceae植物的乾燥根，含系列具生物活性的生物鹼。本文開發一高效液相層析法，可在六十分鐘內順利分析acutumidine（1）、magnoflorine（2）、stepharine（3）、sinomenine（4）、acutumine（5）、cyclanoline（6）、fangchinoline（7）、berbamine（8）、tetrandrine（9）、isotetrandrine（10）、trilobine（11）、aristolochic acid Π（12）、aristolochic acid Ι（13）、aristololactam（14）及isotrilobine（15）等十五成分。該方法以醋酸銨（pH 4.81）及水-氰甲烷為緩衝溶液，可同時適用於高效液相層析及液相層析質譜的分離。層析圖譜（254 nm）上各波峰之化學結構均經層析質譜確認。從台灣、日本、中國收集32批分屬Sinomenium acutumn, Stephania tetrandra, Aristolochia fangchi及Cocculus trilobus的市售藥材，用上述開發的分析方法，可迅速地測出其十五指標成分的含量，這結果可用於防己基原的辨識與藥材品質的評價。

Fangchi Radix is derived from Aristolochiaceae or Menispermaceae plants and contains a series of biologically active alkaloids. Fifteen alkaloids in Fangchi crude extracts, including acutumidine(1), magnoflorine(2), stepharine(3), sinomenine(4), acutumine(5), cyclanoline(6), fangchinoline(7), berbamine(8), tetrandrine(9), isotetrandrine(10), trilobine(11), aristolochic acid Π(12), aristolochic acid Ι(13), aristololactam(14) and isotrilobine(15), were separated completely by high-performance liquid chromatography (HPLC) within 60 min. UV detection at 254 nm with a linear gradient elution consisting of 20 mM CH3COONH4 (pH 4.81) and H2O-CH3CN was found to be the most efficient eluent for the LC and LC-MS separations. The structures of the compounds corresponding to the peaks in the LC chromatogram were identified by LC-MS. A total of 32 commercial samples of Fangchi Radix from Sinomenium acutumn, Stephania tetrandra, Aristolochia fangchi and Cocculus trilobus were collected from herbal markets in Taiwan, Japan and China. The contents (peak-area ratios) and structures of the fifteen constituents in these samples were readily determined by the proposed methods and the results could be used to estimate the origin and quality of an herbal drug.