篇名 | Determination of Glucosamine Content in Nutraceuticals by Capillary Electrophoresis Using In-Capillary OPA Labeling Techniques |
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卷期 | 14:2 |
作者 | JONG-JYH CHEN 、 YEUN CHUNG LEE 、 TZONG-JIH CHENG 、 HSIEN-YI HSIAO 、 RICHIE L. C. CHEN |
頁次 | 203-206 |
關鍵字 | capillary electrophoresis 、 glucosamine 、 nutraceutical 、 in-capillary labeling 、 o-Phthalaldehyde 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200606 |
Capillary electrophoresis (CE) procedure was developed for determining glucosamine content in nutraceuticals. Glucosamine was labeled with o-phthalaldehyde (OPA) by mixing with the OPA reagent (4.5 mM OPA, 4.5 mM 2-mercaptoethanol dissolved in 20 mM borate buffer, pH 9.3). The fluorescent OPA-glucosamine adduct was separated and detected at A340 within 3 min. The pre-capillary labeling reaction can be performed by sequentially injecting OPA/sample/OPA segments into the separation capillary; the in-capillary labeling technique simplified the sample pretreatment and improved its reproducibility. The linear dynamic range of the in-capillary labeling CE method was 0.1~30 mM. No sample pretreatment procedure was required for tablets containing mainly starch or other sugar as the vehicles.