篇名 | 以固相萃取法及氣相層析質譜儀分析尿中大麻代謝物監測大麻濫用者 |
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卷期 | 7:3 |
並列篇名 | Solid-Phase Extraction and Gas Chromatography-Mass Spectrometry Analysis of 11-Nor-9-carboxy-△?-tetrahydrocannabinol in Urine for Monitoring Marijuana Abuse |
作者 | 蔡瑜瑩 、 戴世傑 、 黃明權 、 張柏林 、 廖俊亨 、 劉瑞厚 |
頁次 | 177-184 |
關鍵字 | 氣相層析質譜分析 、 氘內標準品 、 尿中大麻代謝物 、 藥物濫用 、 GC-MS 、 Internal standard 、 11-nor-9-carboxy-△9-tetrahydrocannabinol 、 Urine 、 Drug of abuse 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 199909 |
本報告以固相萃取及甲基衍生化方法來分析尿中大麻代謝物11-nor-9-carboxy-△9-tetrahy-drocannabinol(9-THC-COOH),用以判定受檢者是否使用大麻。d3-9-THC-COOH為內標準品,並以氣相層析質譜儀中之離子監測法(selected ion monitoring)做定性及定量分析。監測9-THC-COOH離子為m/z 313,357,372;d3-9-THC-COOH離子為m/z 316,360,375。5mL空白尿液配製濃度15 ng/mL之9-THC-COOH時,其固相萃取之回收率大於90%。以單點校正方式定量分析,最低可檢濃度為2.09 ng/mL;線性範圍位於4.18 ng/mL-376ng/mL間;而線性範圍內之日間精密度試驗其變異係數(Coefficient of variation)為1.49%-8.03%。
A specimen pretreatment procedure, combining solid-phase extraction andmethylation, was developed for the analysis of 11-nor-9-carboxy-△9-tetrahydrocannabinol (9-THC-COOH) in urine for monitoring marijuana exposure. d3-11-Nor-9-carboxy-△9-tetrahydrocannabinol (d3-9-THC-COOH) was used as aninternal standard, while the now universal selected ion monitoring gaschromatography-mass spectrometry approach was used for detection (m/z 313, 357, 372and 316, 360, 375 for 9-THC-COOH and d3-9-THC-COOH, respectively.) and quantitation(m/z 372 and 375 for 9-THC-COOH and d3-9-THC-COOH, respectively). The effectivenessof the overall procedure was evaluated. One of the extraction columns evaluatedachieved > 90% recovery of the analyte in 5-mL drug-free urine fortified with 15 ng/mL9-THC-COOH. Using the one-point calibration approach, the overall protocol achievedthe following analytical parameters: detection limit: 2.09 ng/mL; limit ofquantitation: 4.18 ng/mL; upper end of linear range: 376 ng/mL (or better); interdayprecision: 1.49-8.03% CV in th 4.18--376 ng/mL concentration range studied.