非法添加於黏膜化妝品的煤焦色素具有強毒性或致癌性，依據我國國家標準(CNS)，化妝品中有機性著色劑之檢驗方法係採用濾紙層析法與薄層層析法。本研究係採用高效液相層析法，尋找最佳條件以分離並鑑別摻加於黏膜化妝品中非法定煤焦色素，以提高檢驗效率及精確度。高效液相層析法條件:層析管為Cosmosi1 5C18-AR-II。移動相:(a)分析16種水溶性煤焦色素使用0.07M醋酸銨內含0.01M溴化四丁基銨:乙腈:甲醇=55:35:10。(b)分析6種脂溶性煤焦色素使用甲醇:水=95:5。偵測器為具有可見光波長之光二極體陣列檢測器(photodiode array detector)。以本法分析22種非法定之煤焦色素均可得到良好之分離效果，最低偵測濃度0.05μg/mL。

　　　　　Illegal coal tar dyes are reported to possess strong toxicity andcarcinogenecity when added in mucous cosmetics. According to CNS, current methodsin analyzing organic coloring materials (dyes) are mainly filter paper chromatographyand thin-layer chromatography. In this study, we adopted a refined HPLC procedurereaching optimal conditions for the separation and identification of the coloringconstituents allegedly added in mucous cosmetics. Ultimately, the analyticalefficiency and precision are greatly improved. The optimal conditions for the HPLCmethod were found with a column of Cosmosil 5C18-AR-II; with the first mobile phaseusing 0.07 M ammonium acetate solution in 0.01 M tetrabutylammonium bromide (CH3COONH4) : CH3CN : CH30H = 55:35:10 for analyzing 16 hydrophilic coal tar dyes, whilethe second mobile phase uses CH30H : H20 = 95:5 for analyzing 6 hydrophobic dyes anda photodiode array detector within UV-VIS wavelength. By applying this HPLC method,a total of 22 banned coal tar dye constituents can be satisfactorily separated. Itis also found that the detection limit can be improved to 0.05 μg/mL.