篇名 | 以高效液相層析法分析包裝水中低分子量醛類含量 |
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卷期 | 11:1 |
並列篇名 | Determination of Low-molecule-weight Aldehydes in Packed Drinking Water by High Performance Liquid Chromatography |
作者 | 蔡佳芬 、 蕭惠文 、 李樹其 、 周薰修 |
頁次 | 46-52 |
關鍵字 | 包裝水 、 甲醛 、 乙醛 、 丙醛 、 DNPH衍生物 、 Packed drinking water 、 Formaldehyde acetaldehyde 、 Propionaldehyde 、 DNPH derivative 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200303 |
A high performance liquid chromatographic (HPLC) method for the determination of formaldehyde, acetaldehyde and propionaldehyde in packed drinking water was developed. Aldehydes were derivatized with 0.5 mL DNPH (2,4-dinitrophenylhydrazine) reagent and 0.1 mL 2 M perchloride at 55°C in 60 minutes, then adsorbed in C18 glass cartridge (was performed), eluted with 5 mL acetonitrile ,and finally determined by HPLC. Formaldehyde, acetaldehyde and propionaldehyde-DNPH-derivatives were seperated on a Cosmosil 5C18-MS column by using acetonitrile/D.I. water (55/45, v/v) as a mobile phase, and detected with UV 360 nm. Recovery studies were performed by fortifying D.I. water with formaldehyde, acetaldehyde and propionaldehyde at various concentrations from 2 to 200 ppb. Recovery rates were in the range of 84.4 – 103.2%, 90.2 – 122.1% and 60.8 – 100.4%. The detection limits for all three aldehydes were 1 ppb. Sixty-three packed drinking water samples collected from various markets were analyzed. These results were well below the guideline for drinking water quality by WHO (900 μg/L).