篇名 | 以聚焦式微波輔助萃取、乙基化及氣相層析質譜法分析魚肉中甲基汞及無機汞含量 |
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卷期 | 12:2 |
並列篇名 | Determination of Methyl- and Inorganic Mercury in Fish Using Focused Microwave Digestion Followed by Cu++ Addition, Sodium Tetraethylborate Derivatization, n-Heptane Extraction, and Gas Chromatography-Mass Spectrometry |
作者 | 陳石松 、 周薰修 、 黃登福 |
頁次 | 175-182 |
關鍵字 | 微波輔助萃取 、 乙基化 、 氣相層析質譜儀 、 魚肉 、 甲基汞及無機汞 、 Methylmercury 、 Inorganic mercury 、 Microwave-assisted digestion 、 Ethylation 、 GC-MS 、 Fish 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200406 |
The analytical procedure for analysis of methyl- and inorganic mercury in fish was developed in this study. It involved microwave-assisted digestion with alkaline solution (tetra methyl ammonium hydroxide), addition of Cu++, aqueous-phase derivatization of mercury species with sodium tetraethylborate, and subsequent extraction with n-heptane. The various mercury derivatives were desorbed in the splitless injection port of a gas chromatograph and subsequently analyzed by electron impact mass spectrometry. Optimum conditions allowed the sample throughout to be controlled by the instrumental analysis time (about 8 min per sample) but not by the sample preparation step. At the irradiation power of 15-30, 45, and 60-75 W, sample heating times were only 3.5, 2.5, and 1.5 min, respectively. The recoveries of mercury species were 92.3–96.1% and 93.6–95.5% for methyl- and inorganic mercury, respectively.The proposed method was finally validated by the analysis of three biological certified reference materials (BCR CRM 464 tuna fish, NRC DORM-2 dogfish muscle, and NRC DOLT-2 dogfish liver).