Reported epidemiology references showed that vaginitis was caused either by bacteria (40-50%), Trichomonas (20-25%) or Candida (15-20%). The objective of this study was to investigate the prevalence and strains distribution of vaginal candidiasis in Taiwan. We evaluate the susceptibilities activity of fluconazole and amphotericin B against 101 Candida isolates from 97 patients suffering from vaginal candidasis. Candida albicans was the most common species (67.3%), followed by C. glabrata (24.7%), C. tropicalis (5.9%), C. parapsilosis (1%), and others (1%) among the identified strains. After evaluation by Etest, a total of 64 (63.4%) and 11 (10.9%) isolates were found to be susceptible, with dose dependent susceptibility, to fluconazole. All Candida isolates were susceptible to amphotericin B with one isolate Saccharomyces cerevisiae being resistant to this drug. Ten out of 68 isolates (14.7%) of C. albicans and 16 out of 25 isolates (64%) of C. glabrata showed fluconazole-resistant. C. glabrata commonly produced higher minimum inhibitory concentrations than all the other species of Candida to triazole agents.