篇名 | A Simple and Fast Method for Arginine Determination in Grape Juice |
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卷期 | 16:3 |
並列篇名 | 離子交換樹脂分離葡萄之中L-精胺酸的快速測定法 |
作者 | 李華 、 梁新紅 、 馮麗丹 、 劉延琳 、 王華 |
頁次 | 53-58 |
關鍵字 | Grape juice 、 Arginine 、 Ion-exchange 、 Ethyl carbamate 、 Sakaguchi reaction 、 葡萄汁 、 L-精胺酸 、 離子交換 、 胺基甲酸乙酯 、 阪口反應 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200806 |
Arginine in grape juice can be metabolized by wine yeasts and malolactic bacteria to precursors of ethyl carbamate, a known carcinogen. The aim of this study was to develop a simple, fast and accurate method for determining arginine in grape juice with Sakaguchi reaction following the separation of arginine with Amberlite IR120 strong cation-exchange resin. Measurement was done under optimized conditions as follows: sample (mg) to cation-exchange resin (g) ratio of 0.683, sample pH 2.0, sample flow rate 0.5 mL/min, use of distilled water rinsing to avoid interference of other compounds adsorbed on the resin, and elution with 1 mol/L of NaOH solution. This method can be applied in a quick and accurate quantification of arginine in grape juice for controlling the ethyl carbamate level in wine.