Methamphetamine (MA) is one of the most commonly abused drugs in Taiwan. In order to investigate the abuse patterns of MA in the Southern Taiwan, urine samples were collected from specified residents of Chiayi City (CCI), Chiayi county (CCO), Tainan City (TCI), Tainan County (TCO), Kaoshiung City (KCI), Kaoshiung County (KCO) and Pingtung County (PCO), representing seven southern cities and counties. A total of 5452 urine samples was collected from July 2006 to December 2006 and subjected to screening for MA with enzyme immunoassay (EIA) and confirmed with GC-MS. As a result, MA positive percent and concentration were 10.9 ± 2.3% and 16535 ± 5682ng/mL, respectively. The positive percent for each location was TCI 14.4 %, CCI 13.2%, PCO 11.3%, KCO 11.2%, CCO 9.0%, TCO 8.7% and KCI 8.7%. The mean concentrations of MA were: TCI 23892, TCO 20566, CCO 17877, KCO 17750, PCO 16260, KCI 13343 and CCI 6058 ng/mL.