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中國園藝

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篇名 出瓶馴化光度與培植期對‘綠巨人’白鶴芋組培苗出瓶生長之影響
卷期 50:1
並列篇名 Effects of Photosynthetic Photon Flux Density during ex vitro Acclimatization and Duration of in vitro Culture on the Growth of Micropropagated Spathiphyllum ‘Sensation’
作者 林怡如葉德銘
頁次 21-30
關鍵字 白鶴芋光度馴化Spathiphyllumacclimatizationphotosynthetic photon flux density
出刊日期 200403

中文摘要

‘綠巨人’白鶴芋組織培養苗出瓶置於25±1℃之育苗室,每日分別處理16小時之35-150 μmol m-2 s-1光度馴化10-30天,並以未經馴化移植置於溫室者為對照組,結果顯示組織培養苗出瓶之適宜馴化光度為100-150 μmol m-2 s-1,其成活率與生長表現均較佳。培植4及14週者出瓶後植株生長較差,以培植6-8週培殖體具有3-4片葉、乾重30-33 mg者較有利出瓶後生長,並可縮短組織培養至出貨時間。培殖體在出瓶移植至無土混合介質後,以葉綠素螢光儀測定瓶內形成葉片之葉綠素螢光值(Fv/Fm),移植後第三天各處理葉片Fv/Fm值呈現下降之趨勢,並於馴化第7天後葉片Fv/Fm值開始回升,至馴化第15-30天,其值為0.78。

英文摘要

Spathiphyllum ‘Sensation’ plantlets were transplanted and placed in a growth room at a temperature of 25±1℃ under cool-white fluorescent light providing 16 h daily photosynthetic photon flux density of 35-150 μmol m-1s-1. After 10 to 30 days of acclimatization in the growth room, the plantlets were transferred to the greenhouse. The optimal irradiances ranged from 100 to 150 μmol m-2 s-1 during acclimatization. The 6 to 8-week-old plantlets in vitro, with 3 to 4 leaves and 30-33 mg dry weights, had greater subsequent ex vitro growth than did the 4 or 14-week-old plantlets. All the Fv/Fm values of in vitro formed leaves decreased on day 3, the value started to increase from day 7 and the Fv/Fm remained at 0.78 from day 15 to 30 after transplanting to soilless mix.

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