以液相層析串聯質譜儀的方法建立黃芩藥材之指紋圖譜。自台灣市場
和原產地收集52個黃芩藥材，再以70%甲醇萃取，高效液相層析條件為
C18分析管柱（150 mm × 4.6 mm, 2.7 µm），移動相為乙腈及0.25%甲酸混
合溶液以流速0.5 mL /min梯度沖提。MS/MS分析係以負離子模式進行全質
量掃描，各個層析峰的紫外線和質譜數據透過標準品以及比對過去的文獻
後共鑑定出16個成分。本方法除了可確認黃芩藥材之正確基原外，並可鑑
別市售製劑辛夷清肺湯、龍膽瀉肝湯及黃連解毒湯中黃芩藥材。

A high-performance liquid chromatography coupled with electrospray ionization (ESI) tandem mass spectrometry (LC/MS/MS)
method was developed for the chemical fingerprint analysis of Scutellaria baicalensis and rapid identification of major compounds in
the fingerprints. The chromatography was carried on a C18 analytical column (150 mm x 4.6 mm, 2.7 µm) with gradient elution using
acetonitrile and 0.25% (v/v) formic acid. Mass spectrometry was performed in the negative ion mode using ESI. Sixteen main peaks in the
fingerprints were identified by comparing the UV and MS spectra data with those from the authentic standards and literature. This method
was successfully employed for the identification of Scutellariae Radix in three traditional Chinese medicine preparations, namely Shin Yi
Ching Fey Tang, Huang Lien Chieh Tu Tang and Lung Tan Hsieh Kan Tang. The developed fingerprint assay was specific and could be
readily utilized for comprehensive evaluation of Scutellariae Radix.