本研究開發並評估一簡易的氣相層析/質 譜儀方法，供定量尿中free codeine，free mor-phine, 6-acetylmorphine, total codeine, total mor- phme。抽取用之緩衝液以pH値為9.0時，其 柚取回收率最高。抽取回收率，free codeine為 98.3%，free morphine 為76,7%，6-acetylmor- phine為85_6%，三甲基矽化衍生物在七十二小時內均非常安定，適合其定量分析。nalor-phine 為內標準品，以 SIM (Selected Ion Monitoring)方式定量。濃度在50至1000 ng/mL範圍內均具有良好的線性關係，日內及 日間精密度試驗顯示，codeine的變異係數為 <147 〜7,72%，morphine 為（X31 〜8,09%，6-acetylmorphine 為 0,49 〜6,15%。

A simple yet effective GC/MS protocol was developed and evaluated for the determination of free codeine, free morphine, 6-acetylmorphine, total codeine and total morphine in urine. Optimal recovery was achieved with extraction conducted at pH 9.0. Trimethylsilyl-derivatives of these analytes and the internal standard (nalorphine) were found adequately stable for a 72- hour period. Quantitative determination of the analytes was performed by selective ion monitoring. Excellent linearity was observed over the 50-1000 ng/mL concentration range studied. The intraday and interday precisions range from 0.47 to 7.72% for codeine, 0.31 to 8.09% for morphine and 0.49 to 6.15% for 6-acetylmorphine. The overall recoveries for codeine, morphine and 6-acetylmorphine were found to be 98.3%, 76.7% and 85.6% respectively.