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藥物食品檢驗局調查研究年報
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| 篇名 | Determination of Polar Constituents of Scrophulariae Radix in Bai-He-Gu-Jin-Tang by Micellar Electrokinetic Capillary Chromatography |
|---|---|
| 卷期 | 17 |
| 作者 | 顧祐瑞 、 周鳳琴 、 溫國慶 、 林哲輝 、 廖俊亨 |
| 頁次 | 251-259 |
| 關鍵字 | Pharmaceutical analysis 、 Scrophulariae Radix 、 Bai-He-Gu-Jin-Tang 、 Harpagoside 、 MEKC 、 TSCI |
| 出刊日期 | 199909 |
中文摘要
英文摘要
A micellar electrokinetic capillary chromatographic method was established for the determination of 2-(3-hydroxy-4-methoxyphenyl)ethyI l-0-[a-L-arabi-nopyranosyl(l—>6)]-feruIoyI(l—>4)-a-L-rhamnopyranosyl(l-^3)-P-D-glucopyra noside (SN-A), harpagoside (SN-B) and cinnamic acid (SN-C) in the traditional Chinese medicinal prescription, Bai-He-Gu-Jin-Tang, which contains Scrophulariae Radix. This chromatographic technique was carried out using a buffer composed of 20 mM sodium tetraborate and sodium dihydrogen phosphate containing 120 mM sodium cholate and 12 % methanol at pH 7.5. 2-(4-Hy-droxyphenyl)-ethylammonium chloride was used as an internal standard and detected at 280 nm. The linear calibration ranges for SN-A, B and C were 11.76-188.16 |lg/mL (r = 0.9998), 8.16-130.56 [ig/mL (r = 0.9992) and 4.48-71.68 ^ig/mL (r = 0.9991), respectively. The recoveries of the markers listed above were SN-A: 104.3 ±2.8 %, SN-B: 98.1 ±2.2 % and SN-C: 98.2 ±1.4 The relative standard deviations of the three marker substances for intraday and interday analyses ranged from 0.85 to 2.87 % and from 1.42 to 4.14 %, respectively.