The theory and method of fuzzy mathematics has been utilized to evaluate comprehensively the status of teachers’ quality construction. Membership function of factors are designed based on the foundation of multi-level model, primary factor set and secondary factor set indicator system together with the weight allocation. According to the actual data of each project in each region, after normalization processing and quantitative calculation, the subproject membership degrees are calculated. The multi-level evaluation matrixes are constructed based on the membership degrees to calculate the multi-level evaluation results and obtain the comprehensive evaluation result finally. The experiment results prove the validity and advantage of the proposed method compared with the traditional methods. A comprehensive evaluation system of teachers’ quality construction in ethnic minority areas has been built based on the method proposed in this paper which has the ability to evaluate comprehensively the status of junior high school teachers’ quality construction in different ethnic minority areas.