本研究提出了一種非軸向掃描多焦平面共軛焦顯微鏡系統，結合了多層體積全像光柵和狹縫陣列檢測，可同時擷取不同深度的光學切片影像。顯微鏡系統中配置多層體積全像光柵，於樣本內的兩個或多個焦平面中選擇性地產生軸向焦點，然後使用共焦狹縫孔徑同時將多個平面成像到 CCD 的感測區域。本文說明了系統設計、實施方法和實驗數據，證明了這種顯微鏡系統不需利用任何軸向掃描裝置即能夠獲得螢光標記的標準微球和組織樣本的光學切片多焦平面影像。

Here, we proposed a non-axial-scanning confocal microscopy. The system is equipped with multiplexed volume holographic gratings for illumination and slit apertures for detection. The multiplexed volume holographic gratings produce multiple axial focal points, and the slit apertures image corresponding axial focal points, which display onto different sensing areas of a CCD. The system design, development and experimental measurements using biological samples are described thoroughly. The proposed system provides optical sectioning using fluorescently labeled standard micro-spheres as well as tissue samples without axial scanning.