本研究以孤挺花(Hippeastrum hybridum Hort.)「紅獅」(‘Red Lion’)與「千禧之星」(‘Blossom Peacock’)組培鱗莖與出瓶苗為材料，探討穀胱甘肽(glutathione)對組培苗瓶內增殖與瓶外生長之影響。利用「紅獅」1/4組培鱗莖於含有不同濃度之還原態穀胱甘肽(reduced glutathione; GSH)或氧化態穀胱甘肽(oxidized glutathione; GSSG)之液態培養基中，於10 μmol m⁻² s⁻¹低照光環境下振盪處理1 h或2 h，再將培植體接續培養於含有3%蔗糖、1.0 mg L⁻¹苯甲基腺嘌呤(benzylaminopurine; BA)與0.1 mg L⁻¹萘乙酸(α-naphthalene acetic acid; NAA)之MS固態培養基中，培養於38 μmol m⁻² s⁻¹照光環境下進行試驗。結果顯示，以65.00 μM GSH溶液振盪處理2 h，再於照光環境下培養12 wk後，每個鱗莖可得14.7苗為最多。將「千禧之星」1/4組培鱗莖培養於含有不同濃度GSH或GSSG液態培養基中，於10 μmol m⁻² s⁻¹低照光環境下進行振盪培養試驗，結果顯示65.00 μM GSH 4 wk培養有助於提高組培苗之增殖效率；延長培養至8 wk時，則GSH與GSSG處理組與對照組之間並無顯著差異；再延長培養至12 wk時，則以16.25 μM GSSG處理組每個組培鱗莖可誘得8.7苗，顯著高於對照組。以鱗莖直徑約12 mm或8 mm之「紅獅」出瓶苗於溫室栽培，並於第9週開始噴施不同濃度穀胱甘肽溶液，而後每4週噴施一次，共處理5次。停止噴施7 wk後，調查植株生長情形。結果顯示大苗鱗莖方面，僅162.80 μM GSSG處理組之葉片鮮重與全株鮮重顯著高於對照組；在小苗鱗莖方面，則以162.80 μM與325.70 μM GSSG處理組之全株鮮重顯著高於對照組。綜合上述結果顯示，「紅獅」培植體以65.00 μM GSH溶液搭配2 h振盪處理，可獲得最高之瓶苗增殖率。「紅獅」組培苗於出瓶8 wk後，每4週噴施一次162.80 μM GSSG溶液，總計噴施處理5次後，可增大鱗莖直徑與提高植株鮮重。

Effects of glutathione on in vitro proliferation and ex vitro growth of plantlets of Hippeastrum hybridum Hort. ‘Red Lion’ or ‘Blossom Peacock’ were investigated in this study. One-quarter in vitro bulb of ‘Red Lion’ was taken as an explant treating with various concentrations of reduced (GSH) or oxidized (GSSG) glutathione for 1 h or 2 h before culturing on a MS solid medium supplemented with 3% sucrose, 1.0 mg L⁻¹ benzylaminopurine (BA) and 0.1 mg L⁻¹ α-naphthalene acetic acid (NAA) for 12 wk under 38 μmol m⁻² s⁻¹ light condition. The best proliferation rate of 14.7 plantlets per bulb was obtained by using 65.00 μM GSH solution for 2 h shaking. In vitro one-quarter bulb of ‘Blossom Peacock’ used as explant was cultured in a liquid medium containing various concentration of GSH or GSSG under 10 μmol m⁻² s⁻¹ light condition for various duration. Result showed that the highest proliferation rates were from 65.00 μM GSH treatment in a 4-wk-span culture and no significant difference was found among all glutathione treatments with the control after 8-wk culturing. However, prolonging culture time to 12 wk, the highest proliferation rate of 8.7 plantlets per bulb was obtained from 16.25 μM GSSG treatment. The effect of glutathione on growth was conducted using the 9-wkold de-flask plantlets of ‘Red Lion’ in either 12 mm or 8 mm bulb diameter by spraying with various concentrations of GSH and GSSG solution every 4 wk for consecutive five times in greenhouse. Growth data of plants was collected at 7 wk after the last glutathione application. Results showed that larger plantlets (with 12 mm bulb diameter) sprayed with 162.80 μM GSSG having higher fresh weight on leaves and whole plant than that of the control. In respect of the smaller plantlets (with 8 mm bulb diameter), higher whole plant weights were found from treatments of 162.80 μM and 325.70 μM GSSG than that of the control. In conclusion, in vitro explants of ‘Red Lion’ treated with 65.00 μM GSH solution for 2 h were found to have the highest proliferation rate. In regard to ex vitro plantlet growth, the 9-wk-old de-flask plantlets of ‘Red Lion’ spraying with 162.80 μM GSSG solution at 4 wk interval for 5 times in total had the largest bulb diameter and fresh weight.