用0.15%的鹽酸甲醇溶液從楊梅（Myrica rubra Sieb. et Zucc.）中提取花色苷以後，先用石油醚和乙酸乙酯分離除去其他多酚類物質，再用C-18小柱純化。楊梅中的花色苷在沒有使用標準對照樣品的情況下，採用了高壓液相色譜（HPLC）-光電二極體陣列（DAD）-電噴霧質譜（ESIMS）聯用和氣相色譜（GC）的方法進行鑒定。楊梅中的花色苷主要為矢車菊色素3-葡萄糖苷，其含量占到了楊梅中總花色苷的97%。該色素可以作為鑒別楊梅加工品真偽的指紋物質，也可以作為鑒別其他植物花色苷中是否存在矢車菊色素3-葡萄糖苷的參照物。在沒有標準樣品時，利用HPLC-DAD-ESIMS和GC的方法可用於研究材料中組分不是很複雜的花色苷的鑒定。

Bayberry (Myrica rubra Sieb. et Zucc.) anthocyanins were extracted with 0.15% HCl in methanol, removed from other polyphenols by partition with petroleum ether and ethyl acetate, and then purified in a C-18 solid phase cartridge. Pigments were analyzed by high-performance liquid chromatography (HPLC), photodiode array detection (DAD), electronic spray ion mass spectrometry (ESIMS) and gas chromatography (GC) without the standard. The major anthocyanin, which contained 97% of the total peak area, was identified as cyanidin 3-glucoside. This pigment may be taken as a marker for authenticity control of bayberry products and has potential as a reference compound of cyanidin 3-glucoside in anthocyanin analysis. When standard chemicals are not available, HPLC-DAD-ESIMS and GC are useful in anthocyanin identification if the anthocyanin profile is not overly complex in the studied materials.