l,4-二氯苯(1,4-Dichlorobenze, 1,4-DCB)為一種廣泛用於除臭和消除霉菌及蛾類的燻煙劑，可能對生產或加工工廠操作員造成某些傷害。1,4-DCB藉由受污染食物與飲水或皮膚接觸進入人體經代謝後，會產生的主要代謝物為2,5-二氯酚(2,5-Dichlorophenol, 2,5-DCP)。與2,5-DCP比較後，1,4-DCB的毒性可反應在人類細胞株上，包含有人類肺癌H1355細胞株、人類肝癌HePG2細胞株、類子宮頸癌HeLa細胞株與人類淋巴癌Jurkat細胞株，這些細胞株屬於1,4-DCB進入人體可能接觸的組織。Trypan blue染色法是常用於30分鐘毒性分析，而caspase3/Pl偵測法則用於檢測細胞凋亡(apoptosis)與細胞壞死(neerosis)。在人類肺癌H1355細胞株、人類肝癌HepG2細胞株、人類子宮頸癌HeLa細胞株與人類淋巴癌Jurkat細胞株毒性結果顯示，l,4-DCB半致死率濃度分別為102、102、142與1.18mM。與1,4-DCB於人類淋巴癌Jurkat細胞株之毒性比較，顯示2,5-DCP之半致死率濃度高達2.03mM。進一步分析人類淋巴癌Jurkat細胞株經1,4-DCB處理24小時之細胞凋亡與細胞壞死結果，顯示1,4-DCB 700µM有較高的細胞凋亡（大約29%）。在HeLa粒線體膜電位偵測方面，500µM 1,4-DCB可造成20%細胞粒線體膜電位喪失。我們證實1,4-DCB與2,5-DCP均具細胞毒性1,4-DCB造成細胞死亡方面包含了細胞壞死與細胞凋亡。

l,4-dichlorobenzene (DCB), used as an ingredient in indoor deodorants, might be hazardous to workers in synthesizing or packaging facilities. 1,4-DCB can be metabolized into 2,5-dichlorophenol (DCP) after entering the human body through the skin or orally via contaminated food and drink. The cytotoxicity of 1,4-DCB, compared with 2, 5-DCP, was examined in human cell lines, including lung adenocaricinoma H1355 cells, hepatoma HepG2 cells, HeLa cells and leukemia Jurkat cells. Trypan blue staining assay was used to detect the cytotoxicity of 30-minute treatment, and caspase/PI detections was used to distinguish apoptosis and necrosis. The LD(subscript 50S) of 1, 4-DCB were 1.02, 1.02, 1.42 and 1.18 mM in lung adenocaricinoma H1355 cells, hepatoma HepG2 cells, leukemia Jurkat cells and HeLa cells. Compared with 1, 4-DCB, 2, 5-DCP had higher LD50 as 2.03 mM in leukemia Jurkat cells. The extent of apoptosis and necrosis in human leukemia Jurkat cells was analuzed after the cells had been treated with 1, 4-DCI3 for 24 hours. A very high percentage of apoptosis was gained (around 29%) in the presence of 1, 4-DCB above 700µM. When apoptosis was analyzed by a mitosensor to measure the mitochondria membrane potential in HeLa cells, 500µM 1, 4-DCB caused 20% of the cells to lose their mitochondria membrane potential.