本研究以高效液相層析法，建立豬肉、豬肝、雞肉、雞肝和雞蛋中氟苯並嘧唑氨基甲酸（flubendazole）殘留量之檢驗方法。檢體以乙萃取，經過過濾，再以乙飽和之正己烷去除油脂、雜質及濃縮後，以Bond Elut C18 樣品處理匣淨化，最後利用高效液相層析儀分析定量。所使用的液相層析管柱為Luna 5μ C18（25 cm × 4.6mm i.d., 5μm），並採用乙與水（7:13, v/v）溶液為移動相溶液，以紫外光檢測器偵測。經添加0.01、0.02 及0.04 ppm flubendazole 標準品之回收試驗，結果，豬肉部份的回收率為92.7∼100.4%，變異係數介於0.8∼2.2%；豬肝的回收率為97.7∼100.4%，變異係數介於0.6∼2.1%；雞肉的回收率為93.9∼99.1%，變異係數介於1.4∼3.4%；雞肝的回收率為91.6∼102.0%，變異係數介於1.4∼5.1%；雞蛋於0.02、0.04 及0.08 ppm 的添加回收率為93.3∼95.3%，變異係數介於0.5∼2.0﹪。本方法檢體的最低檢出限量，除了雞蛋為8 ppb 外，其餘均為4 ppb。將此方法應用於市售豬肉、雞肉之殘留量分析，結果，20 件檢體均未檢出。

A simple and sensitive detection method based on C18 reversed-phase high performance liquid chromatography （HPLC） with UV-detection at 313 nm was presented for the residual determination of flubendazole in swine and chicken muscle, swine and chicken liver, and egg. Flubendazole was extracted from samples with acetonitrile, then filtered. The extract was partitioned with n-hexane, evaporated to dryness, and then cleaned up through C18 cartridge before quantitatively determinated by HPLC. Flubendazole was analyzed by HPLC on a column of Luna 5C18（25cm × 4.6 mm i.d., 5m） using acetonitrile/ water（7/13，v/v） as eluent，and determined by UV detector. The average recoveries of flubendazole spiked into samples at the levels of 0.01, 0.02, and 0.04 ppm were in the range of 92.7∼100.4﹪for swine muscle，97.7∼100.4﹪for swine liver，93.9∼99.1﹪for chicken muscle，91.6∼102.0﹪for chicken liver respectively. Similarly spiking into egg at the levels of 0.02, 0.04, and 0.08 ppm were in the range of 93.3∼95.3﹪. Coefficients of variation were less than 5.1﹪. The detection limits were 4 ppb in all tissue samples but was 8 ppb for egg. No residue of flubendazole was detected in twenty samples which were purchased from various supermarkets in Taiwan.