In this study, a rapid and simple method without a derivatization process fordetermination of the free sterols in vegetable and animal oils was developed. A directinjection gas chromatography with a non-polar megabore column (DB-1, 5 m x 0.53mm, 1μm) was used. The recoveries of cholesterol, campesterol, stigmasterol and β-sitosterol from lard were at the range of 89%-106% with a coefficient of variationless than 12%. Total sterols were determined using the same method aftersaponification of the oil sample. Fourteen samples, including olive oil, sunflower oil,peanut oil, soybean oil, sesame oil, lard and egg yolk oil, were quantitatively analyzedfor total and free sterols. The contents of total cholesterol, brassicasterol, campesterol,stigmasterol, β-sitosterol,Δ5-avenasterol,Δ7 -stigmastenol and Δ7-avenasterol intest samples were at the range of 193-34384, 0-1222, 0-2356, 0-1365, 0-5414, 0-608,0-1296 and 0-129 μg/g, respectively; while the contents of free cholesterol,brassicasterol, campesterol, stigmasterol, β-sitosterol, Δ5-avenasterol, Δ7-stigmastenol and Δ7-avenasterol were 98-30340, 0-1057, 0-634, 0-724, 0-3087, 0-404, 0-558 and 0-23 μg/g, respectively. The amounts of esterified sterols werecalculated by subtracting the free sterol contents from total sterol contents. Resultsshowed olive oil, sunflower oil and peanut oil primarily contain esterified sterols;while soybean oil, sesame oil, lard and egg oil contain mostly free sterols. Thedeveloped method is recommended as a rapid method to determine if animal fats/oilsare adulterated with vegetable oils.