本研究利用高效液相層析法(high performance liquid chromatography，HPLC)分析紙製容器 (餐盤、紙杯及便當盒 ) 中之螢光增白劑 (fluorescent whitening agents)。經依據衛生署公告第 762034 號之螢光增白劑檢驗方法，將紗布浸泡後，以二甲醯胺萃取，經減壓濃縮至乾，轉溶後再以 HPLC 分離鑑定。發現螢光增白劑可以下列之 HPLC 分析條件加以分離鑑定， 即以 Prodigy 5 um ODS (2) 為分離管， 乙腈:0.01 M 磷酸二氫鈉(35:65，v/v) 為移動相，以螢光檢測器於激發波長 340 nm 及放射波長 430nm 檢測。其次，以上述條件，分析五十件市售紙製容器，經確認結果均未檢出螢光增白劑。

　　　　　The method of determination of fluorescent whitening agents (FWA) inpaper containers for food was studied. Samples were assessed in accordance withofficial procedures. The treated gauze was extracted by dimethylformamide,evaporated to dryness, and then analyzed by HPLC. The extracted FWAs wereseparated with Prodigy 5 um ODS (2)column using acetonitrile: 0.01 M sodiumdihydrogen phosphate (35:65) as solvent. For spectrofluorimetric detection, anexcitation wavelength of 340 nm and emission wavelength of 430 nm were used.Applying the above HPLC method, fifty samples of paper containers for food wereanalyzed. No residue of FWA was detected in any sample.