本文提供一簡單、快速之逆相高效液相層析法,可同時鑑別十種感冒藥:acetaminophen、 bromvalerylurea、 bucetin、caffeine、chlorpheniramine maleate、dextromethorphan HBr、 ethoxybenzamide、 guaifenesin、 methylephedrine HCl 及noscapine HCl 等成分。 本法採用之分析管柱為 Inertsil ODS-3V column (5 um, 250 x4.6 mm), 移動相組成為含 2 % tetrahydrofuran、 0.2 % triethylamine 之 0.1 % H3PO4 水溶液:CH3CN(85:15) 混液, 以磷酸調整 pH 至 2.70, 移動相之流速為1.0ml/min,偵測器係利用紫外光分光光度計,波長設至 205 nm。 本法以 bucetin 為內部標準品,可以於 50 分鐘內完成上述十種感冒藥成分之分離,經確效結果,分離率 (Rs) 為1.63 ~ 9.08,其標準曲線線性良好、精密度及準確度頗佳,可應用此方法分析、定量市售檢體,足見其實用性。
A simple, rapid and reliable high performance liquid chromatographicmethod for simultaneous determination of compounds typically found in complexcold medicines (i.e. acetaminophen, bromvalerylurea, bucetin, caffeine,chlorpheniramine maleate, dextromethorphan HBr, ethoxybenzamide, guaifenesin,methylephedrine HCl, noscapine HCl) has been developed. Isocratic separation wasperformed using an Inertsil ODS-3V column (5 um, 25 cm x 4.6 mm i.d.)with amobile phase:0.2% triethylamine, 2% tetrahydrofuran in 0.1% H3PO4:CH3CN(85:15; pH=2.70), flow rate 1.0 ml/mmin. Au ultraviolet spectrophotometer wasused as the detector at the wavelength of 205 nm. The precision and accuracywere satisfactory. The system can be used to assay the above compounds attypical cold medicine dosage forms.