本研究使用等壓高效液相層析法，同時分析黃芩傳統煎劑及濃縮製劑中之黃酮類成分 -- 黃芩苷、黃芩苷元和漢黃芩素之含量。 採用 Inertsil ODS-2 逆相層析管柱， 以acetonitrile-0.005 ％ phosphoric acid (36:64，v/v) 之混合溶液為移動相， 流速為 1mL/min，檢測波長為 270 nm，並以 ethyl paraben 為內標準。此分析方法可應用於黃芩藥材和濃縮製劑產品之品質管制。定量分析各十個市場品之結果顯示，每公克黃芩之傳統煎劑含黃芩苷、 黃芩苷元及漢黃芩素平均分別為 62.7 ± 4.6、5.5 ± 0.2 及 1.7 ± 0.1 毫克，而濃縮製劑中每公克平均含 82.3 ± 8.5、15.0 ± 2.0 及 5.3 ± 0.5 毫克。計算其每日最大劑量，黃芩傳統煎劑平均含黃芩苷、黃芩苷元及漢黃芩素分別為 564.7 ± 41.7、49.7 ± 1.9 及 15.6 ± 1.0 毫克， 而濃縮製劑則含 241.9 ± 41.1、 53.7 ± 16.4 及17.9 ± 4.6 毫克。

　　　　　Baicalin, Baicalein and wogonin are bioactive flavonoids ofScutellariae Radix. In order to compare the contents of baicalin. baicalein andwogonin between traditional decoction and commercial extract, ten crude drugsand ten commercial extracts of Scutellariae Radix were purchased from themarket. High performance liquid chromatographic method was used for simultaneousassay of these flavonoid constituents. The separatin was performed on anInertsil ODS-2 column with acetonitrile -0.005% phosphoric acid (36:64, v/v) asmobile phase at a flow-rate of 1.0 mL/min and detection at 270 nm, and ethylparaben was used as the internal standard. This method is applicable for theroutine quality control of the crude drug and commercial extract of ScutellariaeRadix. The results indicated that the contents of baicalin, baicalein andwogonin in traditional decoctions of each gram of Scutellariae Radix were 62.7± 4.6 mg, 5.5 ± 0.2 mg and 1.7 ± 0.1 mg, whereas those in each gram ofcommercial extracts were 82.3 ± 8.5 mg, 15.0 ± 2.0 mg and 5.3 ± 0.5 mg,respectively. The suggested daily doses of crude drug and commercial extract ofScutellariae Radix are 3.0-9.0 g and 0.4-9.0 g, respectively. Based on ourresults, the contents of baicalin, baicalein and wogonin in the maximum dailydoses for traditional decoctions of Scutellariae Radix were 564.7 ± 41.7 mg,49.7 ± 1.9 mg and 15.6 ± 1.0 mg, whereas those for commercial extracts were241.9 ± 41.1 mg, 53.7 ± 16.4 mg and 17.9 ± 4.6 mg, respectively.