一種來自東南亞的中草藥檢體，以薄層層析及紫外分光光度計檢驗出含prednisone 成分，經固相萃取管處理後，再以高效液相層析法定量。 固相萃取管依序以甲醇及氯仿先活化，繼用二氯甲烷及異丙醇 (6：4, v ╱ v) 沖提檢體。高效液相層析採用逆相層析管注 (Inertsil ODS-80A)，沖提液為乙　與水 (3：7，v ╱ v)，檢測波長為 240nm，內部標準品為 fludrocortisone acetate。Prednisone 檢量線之濃度範圍為 10.0-200.0μ g/mL。 確效結果顯示精密度、準確度及回收率均良好。以上述方法定量分析檢出每包檢體 (約 21 公克 ) 含 prednisone 5.5 毫克。

　　　　　Prednisone was detected in a herbal medicine from Southeast Asia usingthin layer chromatography (TLC) and ultraviolet spectroscopy (UV). Aftertreating with solid-phase extraction (SPE), the sample was further assayed byhigh-performance liquid chromatography (HPLC). The sample solution was achievedwith a silica gel cartridge conditioned with methanol and chloroform insequence, and then eluted with dichloromethane-isopropanol (6: 4, v/v) .Separation was conducted with an Inertsill ODS-80A reversed-phase column usingisocratic elution with acetonitrile and water (3:7, v/v) as mobile phase.Fludrocortisone acetate was used as an internal standard and detectionwavelength was at 240 nm. Calibration curve of standard prednisone wasconstructed in the range of 10.0-200.0 μ g/mL. The content of adulteratedprednisone was 5.5 mg/21g sample.