Products of fermentation, especially volatile compounds, and the carbon balance of a spoilage microorganism usually provide useful
information to understand the fermentation characteristics of the organism and as a basis for determination of contaminants. In this study,
the cultivation of spoilage Bacillus cereus at 35°C in a culture medium with glucose as the sole carbon source was performed in order to
measure fermentation products including headspace gases, and overall carbon balance during fermentation. The organism reached their stationary
phase after 20 h growth. Under anaerobic fermentation, the recovery ratio of carbon was higher than the corresponding aerobic one.
The major fermentation products were 2,3-butanediol and ethanol under anaerobic fermentation, and it's 2,3-butanediol under anaerobic
fermentation. The calculation of the balance of oxidation and reduction reaction yielded a theoretical value nearly equal to 1 during anaerobic
fermentation. The results of this study can support to develop a system for rapid detection of headspace gases of major fermentation
products specific to food spoilage by using a gas biosensor.