篇名 | 利用微透析聯結高效能液相層析測定茶及茶品中之兒茶素 |
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卷期 | 11:4 |
並列篇名 | The Application of On-line Microdialysis with High Performance Liquid Chromatography for Determining Catechins in Tea and Tea Drinks |
作者 | 楊慶成 、 張嘉真 |
頁次 | 302-306 |
關鍵字 | 微透析 、 高效能液相層析 、 茶 、 HPLC 、 Microdialysis 、 Epicatechin 、 Epicatechin gallate 、 Epigallocatechin 、 Epigallocatechin gallate 、 Tea 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200312 |
A microdialysis on-line coupled to a reversed-phase liquid chromatography was investigated and applied for analyzing catechins in tea and tea drinks. Catechins in drink samples were diffused into the perfusate through a hollow fiber membrane and then on-line injected into the HPLC system. Catechins were separated with a C8 reversed column by gradient eluting using an acetonitrile/ phosphate buffer solution at pH 4.0, and monitored by a UV diode-array detector. Factors affecting the dialysis efficiency such as the conditions in perfusate and in the sample solution were evaluated. The proposed method provided a simple procedure for isolating catechins from tea and tea drink samples. The application was illustrated by the analysis of epicatechin, epicatechin gallate, epigallocatechin, and epigallocatechin gallate in tea drinks.