篇名 | 以高效能液相層析法同步測定臺灣產山藥中之Furostanol和Spirostanol Glycosides |
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卷期 | 11:4 |
並列篇名 | Simultaneous Determination of Furostanol and Spirostanol Glycosides in Taiwanese Yam (Dioscorea spp.) Cultivars by High Performance Liquid Chromatography |
作者 | 楊登傑 、 呂廷璋 、 孫璐西 |
頁次 | 271-276 |
關鍵字 | 高效能液相層析法 、 山藥 、 皂素 、 蒸發激光檢測器 、 薯蕷皂配基 、 Diosgenin 、 Evaporative light scattering detector 、 Furostanol glycoside 、 High performance liquid chromatography 、 Saponins 、 Spirostanol glycoside 、 Yam 、 Dioscorea spp. 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200312 |
A high performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of the steroid saponins, furostanol and spirostanol glycosides in Taiwanese yam cultivars. C18 column kept at 45ûC was used as the stationary phase. The mobile phase was a binary solvent system consisting of methanol and deionized water (62/38, v/v) in the first 20 min and 71/29 (v/v) from 21 to 65 min at a flow rate of 1 mL/min. Both evaporative light scattering detector (ELSD) and UV (203 to 215 nm) were employed and compared for the detection of saponins. The detection limits for ELSD and 203 nm UV were 2~3.5 mg and 0.1~0.2 µg, respectively at signal-to-noise ratio of 10. The recoveries of furostanol and spirostanol glycosides in the range of 0.25~3 µg were all above 92%. The presence of steroid saponins in Taiwanese yams was also determined.