篇名 | 應用毛細管電泳線上樣品堆積法於柴胡皂?分析 |
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卷期 | 11:3 |
並列篇名 | Separation of Saikosaponins by On-line Sample Stacking CE Method |
作者 | 盛珮雯 、 黃文盈 、 許順吉 |
頁次 | 209-213 |
關鍵字 | 毛細管電泳掃集法 、 柴胡皂? 、 柴胡藥材與製劑 、 CE-sweeping method 、 Saikosaponins 、 Bupleuri radix and its preparations 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200309 |
Saikosaponins a, c, d, b1 and b2 which are the trace constituents in the crude extract of Bupleuri Radix can be separated successfully by the on-line sample stacking technique (Sweeping method) of capillary electrophoresis. The method uses aqueous phosphoric acid with a pH lower than 2 to prepare the extracting solvent and buffer solution of the same electric conductance. The former is prepared with methanol and the latter is prepared by adding SDS, methanol and acetonitrile. Bupleuri Radix extract analyzed by CE-sweeping method gives better result than by MEKC method with a higher separation efficiency, lower detection limit and ability to detect five saikosaponins. Applied to the analysis of two Chinese herbal formulas, CE sweeping method is found to be able to determine the contents of saikosaponins a and b1 successfully in Minor Bupleurum Combination and Major Bupleurum Combination, both of which contain Bupleuri Radix as the imperial drug. However, the migration times and resolution of the two marker substances, saikosaponins a and b1, were changed obviously by the complicate constituents in Chinese herbal preparations.