篇名 | 松杉靈芝三?類成分靈芝酸之定量 |
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卷期 | 11:3 |
並列篇名 | Determination of Ganoderic Acids in Triterpenoid Constituents of Ganoderma Tsugae |
作者 | 陳登海 、 陳光地 |
頁次 | 195-200 |
關鍵字 | 靈芝 、 松杉靈芝 、 三?類 、 靈芝酸 、 Ganoderma 、 Ganoderma tsugae 、 Triterpenoid 、 Ganoderic acid 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200309 |
Ganoderma product has the highest sale volume in the health food market in Taiwan. The most widely-used raw material is the species of G. tsuage. Nine triterpenoids were isolated from the fruiting body of G. tsugae and their structures were identified, using 1H NMR and FT-IR, as ganoderic acid A (1), B (2), C (3), D (4), E (5), C5 (6), C6 (7), G (8) and ganoderenic acid D (9). A reverse-phase HPLC method was also established for the determination of these nine triterpenoids. Among these compounds, ganoderic acid E, C5, C6, G and ganoderenic acid D were analyzed for the first time by reverse-phase HPLC. The separation was carried out on a C18 reverse-phase column with a gradient elution of acetonitrile and 2% acetic acid (1/4 and 1/2, v/v) as mobile phase at a flow-rate of 0.8 mL/min and detection at 252 nm. Regression equation revealed the linear relationship (correlation coefficient: 0.9990~0.9999) between the peak-area ratios and concentrations of ganoderic acids. The recoveries of nine ganoderic acids were between 96.85~105.09%. The relative standard deviations of nine ganoderic acids range from 0.8~4.8% (intraday) and 0.7~5.1% (interday). The total contents of the nine ganoderic acids in eight Ganoderma samples were between 0.28~2.20%.