篇名 | 非固醇類抗炎劑之光分解(2)--以液相層析-質譜法連線測定卡普洛芬光分解產物 |
---|---|
卷期 | 11:3 |
並列篇名 | Photolysis of NSAIDs (Ⅱ)--Online LC-MS Determination of Photodegradants from Carprofen |
作者 | 陳福安 、 王博譽 、 溫國慶 、 陳朝洋 、 吳安邦 |
頁次 | 186-190 |
關鍵字 | 光分解 、 卡普洛芬 、 非固醇類抗炎劑 、 液相層析-質譜法 、 去氯基反應 、 Photolysis 、 Carprofen 、 NSAIDs 、 LC-MS 、 Dechlorination 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200309 |
Carprofen in a 10% aqueous ethanol solution at a concentration of 0.10 mg/mL was subjected to photo-irradiation under sunlight for 24 hr. Four photodegradants were separated and subsequently followed an online determination of their quasi molecular ions using an LC-MS method. The HPLC consisted an Inertsil 5 ODS-80A (2.1 mm i.d. × 150 mm) column, and the mobile phase was initially CH3CN: NH4OAc (20 mM in D.I. H2O) = 43: 57 (v/v). After 14 min, CH3CN: NH4OAc (20 mM in D.I. H2O) was changed to 54: 46 (v/v). The UV detector was set at 260 nm. The parameters of LC-MS for mass determination were optimized with an API electron spray interface with negative mode of polarity (ESI). The chemical structures of the degradants were elucidated based on the m/z of the quasi molecular ions. Two degradants were found to proceed via an initial dechlorination with C-Cl cleavage. Dechlorination was observed to be competing with decarboxylation, i.e. either reaction is in accord with our previously reported result with a first-order photodecomposition kinetics of carprofen.