篇名 | 攜帶雞卵蛋白聚乳酸甘醇酸微粒固化過程中二氯甲烷揮發特性之探討 |
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卷期 | 12:4 |
並列篇名 | Dichloromethane Evaporative Behavior during the Solidifying Process of Ovalbumin-Loaded Poly (DL Lactic-co-Glycolic Acid) Microparticles |
作者 | 陳錦龍 、 葉明功 、 江樵熹 |
頁次 | 291-298 |
關鍵字 | 聚乳酸甘醇酸 、 二氯甲烷揮發 、 氣相層析 、 鹽效應 、 PLGA 、 Gas chromatography 、 Salt effect 、 Dichloromethane evaporation 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200412 |
The purpose of this study is to develop an analytical method for determining the evaporative behavior of dichloromethane (DCM) during the solidifying process of poly(DL lactic-co-glycolic acid) (PLGA) in the preparation of ovalbumin-loaded microparticle (OVAMP) with double emulsion solvent extraction method. The time courses of DCM levels in the external phase and total mixture were determined by a gas chromatography (GC) method combined a headspace sampler. Samples were spiked with an internal standard carbon tetrachloride, prior to mixing with a large volume of chloroform. The retention times of carbon tetrachloride, DCM and chloroform were 2.6, 2.8 and 3.3 min, respectively. The analytical method had a minimum quantitative concentration of DCM 7.3 mM and good linearity from 7.5 to 75 mM with coefficient of variations 1.2~3.9%. Four formulations containing NaCl (0~5%) and urea, an osmotic agent to adjust osmolarity at 1240 mOsm/kg, were investigated. The disappearance of DCM levels in total mixtures of four formulations were described as a function of time by zero-order (0~15 min) and first order (after 15 min) kinetics. During the initial 15 min, these formulations had almost identical zero-order rate constants, 2.30~2.63 mmole/min. After 15 min, formulation with 5% NaCl(F4) showed a significant lower value of rate constant (0.193 min-1) in comparison with other formulations using the lower amounts of NaCl (0.288~0.367 min-1). It indicated that the surface characteristic of the F4 OVA-MP was different from other formulations by forming a crust-like structure to inhibit the efflux of DCM from the inner layer. In conclusion, we established a rapid and convenient GC approach without the need for sample pre-treatment for determining DCM levels during the solidifying process of microparticles. The analytical method could be applied to further assess the relationship between DCM level and formulation factors of drug-loaded microparticles.