篇名 | 利用高效液相層析法定量美白化?品中Magnesium Ascorbyl Phosphate、Ascorbyl Glucoside、Kojic Acid、Arbutin及Hydroquinone等成分 |
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卷期 | 12:1 |
並列篇名 | Simultaneous Determination of Magnesium Ascorbyl Phosphate, Ascorbyl Glucoside, Kojic Acid, Arbutin and Hydroquinone in Skin Whitening Cosmetics by HPLC |
作者 | 黃守潔 、 林澄琴 、 黃明權 、 溫國慶 |
頁次 | 13-18 |
關鍵字 | 高效液相層析法 、 美白化?品 、 Magnesium ascorbyl phosphate 、 Ascorbyl glucoside 、 Kojic acid 、 Arbutin 、 Hydroquinone 、 Whitening cosmetics 、 HPLC 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200403 |
A high performance liquid chromatographic method was developed for simultaneous determination of 5 whitening ingredients: magnesium ascorbyl phosphate, ascorbyl glucoside, kojic acid, arbutin and hydroquinone decomposed from arbutin in cosmetics. Samples were extracted with 0.05 M KH2PO4 buffer solution (pH 2.5) and analyzed using a Cosmosil 5 C18-AR-II column. A mixture of 0.05 M KH2PO4 buffer solution (pH 2.5) and methanol (99:1, v/v) was used as mobile phase. The UV detector was set at 280 nm. Pyridoxine was used as an internal standard. The related coefficients, R2, of regression equations of the 5 standard curves were 0.9998- 1.0000. The relative standard deviations of the 5 ingredients for intraday and interday analysis were less than 2.4%. The average recoveries of these 5 ingredients spiked in sample ranged from 93.5% to 103.3%. The relative standard deviations of average recoveries were less than 1.3%. The limits of quantitation in cosmetics were 80.0, 20.0, 3.0, 15.0 and 10.0 μg/mL for magnesium ascorbyl phosphate, ascorbyl glucoside, kojic acid, arbutin and hydroquinone, respectively.