篇名 | 植物雌激素對細胞增生、細胞週期進行和雌激素受器Alpha的綜合效應 |
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卷期 | 13:1 |
並列篇名 | The Combination Effects of Phytoestrogens on Cell Proliferation, Cell Cycle Progression and Estrogen Receptor Alpha |
作者 | 應靜雯 、 徐濟泰 、 楊正大 、 黃新傑 、 王律凱 、 林俊銘 、 陳榮芳 |
頁次 | 30-37 |
關鍵字 | 植物雌激素 、 生長 、 雌激素受器 、 Phytoestrogen 、 Fertility 、 Estrogen receptor 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200503 |
Phytoestrogens are plant chemicals that have been related to the occurrence of human breast cancers and other estrogen-dependent cancers. This study was designed to examine the potential combinational effects and estrogenic activity of phytoestrogens in MCF-7 human breast cancer cells. Results showed that the phytoestrogens biochanin A and daidzein inhibited the proliferation of MCF-7 cells in a dose-dependent manner. Both biochanin A and daidzein delayed the progression of the cell cycle from the G2/M phase to the G1 phase at growth inhibitory concentrations. Treatment with biochanin A down-regulated the level of estrogen receptor-alpha (ER-α) mRNA, but this down-regulation was not affected by the presence of the antiestrogen ICI182,780. When cells were treated with biochanin A at a growth inhibitory concentration of 40 μg/mL, co administration of daidzein resulted in an enhanced inhibitory effect on the MCF-7 cell growth. In addition, neither the growth inhibition, nor the delayed cell cycle progression induced by biochanin A was affected by the addition of antiestrogen. These results suggest that the growth regulatory effects of phytoestrogens may be associated with cell cycle progression, and the underlying mechanism involves at least in part the modulation of a non-classical ER-α signal pathway.