篇名 | 以毛細管電泳分析蜂膠中的類黃酮物質 |
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卷期 | 13:1 |
並列篇名 | Analysis of Flavonoids in Propolis by Capillary Electrophoresis |
作者 | 傅素鄉 、 楊明華 、 溫惠美 、 陳景川 |
頁次 | 43-50 |
關鍵字 | 蜂膠 、 類黃酮 、 毛細管區帶電泳 、 膠束動力學毛細管層析 、 Propolis 、 Flavonoids 、 Capillary zone electrophoresis 、 Micellar electrokinetic capillary electrophoresis 、 MEDLINE 、 Scopus 、 SCIE |
出刊日期 | 200503 |
Flavonoid contents of nine raw and nine commercial propolis samples were analyzed by capillary electrophoresis. At first, 15 commercially available flavonoid standards including apigenin, chrysin, luteolin, galangin, kaempferol, morin, myricetin, quercetin, quercitrin, rutin, hesperetin, naringin, naringenin, daidzein and genistein were used to develop the electrophoretic conditions. At a running temperature of 25°C and detection wavelength at 214 nm, the separation of these standards was most satisfactorily achieved by a combination of capillary zone electrophoresis (CZE), utilizing 0.1 M borate buffer (pH 9.5) containing 5% methanol under a voltage of 18 kV, and micellar electrokinetic capillary electrophoresis (MECC), utilizing 0.03 M sodium borate (pH 8.5) containing 0.05 M SDS under a voltage of -15 kV. We found 12 out of the 15 flavonoids were identified in raw propolis, but only 11 were detected in commercial propolis products. The total contents of the identified flavonoids in raw and commercial propolis were 254 to 19147 ppm and 1228 to 7985 ppm, respectively, suggesting that both the origin and processing are important factors affecting the flavonoid content in propolis.