本研究以PCR 方法探討鑑別檢測基因改良大豆與基因改良玉米之可行性。針對Roundup Ready
（Monsanto 公司）GM －大豆及Event 176（Novartis/Ciba-Geigy 公司）GM －玉米產品插入基因與品種特性基因選定不同引子，進行PCR 方法檢測。用以鑑別GM －大豆之引子共四對，分別為35S（35S-promoter, 源自cauliflower mosaic virus）、NOS（nopaline synthase-terminator,源自Agrobacterium tumefaciens）、EPSPS（5-enolpyruvylshikimate-3-phosphate synthase ，源自A. tumefaciens strain CP4）及LE（品種特性基因lectin）。而鑑別GM －玉米之引子則採用三對，分別為CDPK-cry (pollen-specific calciumdependent protein kinase promoter -delta-endotoxin ，分別源自玉米及Bacillus thuringiensis subsp. kurstaki）、cryIA(b)（delta-endotoxin，源自B. thuringiensis subsp. kurstaki）及ivr（品種特性基因invertase）。結果顯示，大豆檢體以35S 及EPSPS 引子檢測時，其最低檢測量均為0.1%（w/w），NOS 則為1%（w/w）；檢體並以LE 引子－ PCR 反應確定均為大豆產品。至於玉米檢體之測試結果，使用CDPKcry引子其最低檢測量為0.1%（w/w）， cryIA(b)為2%（w/w），並經由Invertase 引子－ PCR 反應確定為玉米產品。此外，GM －大豆之35S-PCR 產物，進一步以限制酵素Xmn I 進行切割確認， 195 bp 產物切成80 bp 與115 bp 產物，而NOS 之180 bp 產物則以限制酵素Nsi I切為84 bp 與96 bp，確認PCR產物。結果顯示本報告所使用之PCR 方法能區分一般與基因改良之大豆及玉米。

　　　　　The feasibility of detecting genetically modified (GM) soybeans and GMmaize by a polymerase chain reaction (PCR) method is determined. Primers specificfor inserted genes and crop endogenous genes in Roundup Ready soybeans(Monsanto company) and Event 176 GM maize (Novartis/Ciba-Geigy company) wereapplied. Four pairs of primers, namely, 35S (35S-promoter, originated fromcauliflower mosaic virus), NOS (nopaline synthase-terminator, derived fromAgrobacterium tumefaciens), EPSPS (5-enolpyruvylshikimate-3-phosphate synthase,obtained from A. tumefaciens strain CP4) and LE (endogenous gene lectin) were usedto identify the GM soybeans. An additional three pairs of primers, including CDPK-cry (pollen-specific calcium-dependent protein kinase promoter - delta-endotoxin,acquired from maize and Bacillus thuringiensis subsp. kurstaki, respectively), cryIA(b)(delta-endotoxin, evolved from B. thuringiensis subsp. kurstaki) and ivr (endogenousgene invertase) were directed to confirm the GM maize. Using 35S and EPSPS asprimers, the method showed a limit of detection for samples containing 0.1% (w/w) ofGM soybeans and containing 1% (w/w) of GM soybeans when NOS primers wereapplied. All soybean samples were evidenced by LE primer-PCR as soybean products.Detection limits of 0.1% (w/w) of GM maize in raw material using CDPK-cry primersand 2% (w/w) of GM maize with cryIA(b) were established. The maize products werealso approved by Invertase primer-PCR. To further confirm detection of the targetGM soybean by PCR, the 195 bp fragment, amplified from 35S-PCR, digested withendonuclease XmnI resulted in 80 and 115 bp fragments, while digesting the 180 bpamplified products from NOS-PCR using endonuclease NsiI yielded 96 and 84 bpfragments. The data further revealed that the PCR method can sufficientlydifferentiate GM soybeans and maize from non-GM products.