本研究於民國86年1月至6月間，針對臺灣區上市前之豬屠體進行抗生素殘留情況之調查；經臺北縣、臺中縣、高雄縣等19個縣市之家畜疾病防治所檢驗員至肉品市場採豬屠體共1022件，以改良式四種培養基檢測法做初步篩檢肉中抗生物質殘留情形。改良式四種培養基檢測法係檢測生物制菌圈，四種培養基中有二種培養基菌層為枯草桿菌，其pH分別為6.0及8.0，第三種培養基菌層為仙人掌桿菌，第四種培養基菌層為卵黃色八聯球菌。生豬肉中四環素類抗生素殘留以生物分析法、生物自析鑑別法定量及定性分析。另從1022件樣品中隨機抽樣以酵素兔疫分析方法檢測鏈黴素、氮黴素，再以HPLC方法定量。檢測結果豬肉抗生物質陽性率0.1%，四環素類殘留率4.11%，氮黴素殘留率0.64%，鏈黴素殘留率0%。由本研究顯示出改良式四種培養基檢測法為一種不用昂貴儀器或複雜方法，比目前臺灣以CNS5916方法或歐聯新近發展之四種培養基檢測法可檢出更多之抗生素及更靈敏之感度，故建議改良式四種培養基檢測法可取代目前CNS5916方法或歐聯之四種培養基檢測法。

　　　　　The antibiotic residues in 1,022 pork carcass samples, which werecollected from 19 areas in Taipei, Taichung and Kaohsiung counties betweenJanuary and June, 1997, were systematically investigated using a modified four-plate test (NFPT). Four culture media inoculated with 3 strains of bacteria wereused; two were inoculated with Bacillus subtilis at pH 6.0 and pH 8.0, and thethird and fourth were inoculated with Bacillus cereus and Sarcina lutea, respec-tively. The identification and quantification of tetracycline residues in theraw meat was performed by bioassay and bioautography. Streptomycin andchloramphenicol in pork carcasses were screened by ELISA and further quantifiedusing the HPLC method. Results showed that using the CNS 5916 method, only I outof 1,022 test samples (0.1%) was detected to contain an antimicrobial agent.While using the MFPT method, 4.11% and 0.64% test samples were found to containtetracycline and streptomycin residues, respectively. No chloramphenicol residuewas detected in any tested samples. It was concluded that the MFPT method ispractical, sensitive, less expensive, and simple as compared to the CNS 5916 andFPT methods, which are routinely used in Taiwan and the European Community,respectively. MFTP is therefore recommended as an official method to assay theantimicrobial agents in foods.