使用PCR之普遍性引子增殖酵母菌之核糖體RNA基因之內轉錄區，以檢測單一或混合二種或三種酵母菌之空敏度。以Novozyme分解三個屬之酵母菌的細胞壁抽取DNA，DNA的抽取量隨茵種不同而有很大的差異。以一對普遍性PCR引子增殖單一和混合酵母菌的核醣體RNA基因的內轉錄區ITS I，PCR之靈敏度在Saccharomyces exiguus為2.5pg的抽取DNA(約10 2個細胞)，Candida mogii為12pg(約10 3個細胞)，Saccharomyces cerevisae為20pg(約10 3個細胞)，由於三株菌經由PCR增殖產物的片段長度皆不同，因此以PCR增殖二種或三種混合酵母菌之DNA，在同樣PCR條件下，其靈敏度較單一酵母菌降低約1000倍(約10 5 ～ 10 6 個細胞)。

To detect the sensitivity of PCR using universal primers to amplify the RNA gene of internal transcribed spacer (ITS I) with a mixture of yeast species. The amounts of extracted DNA obtained from three yeast genera using Novozyme to lyse the cell walls differed significantly. When a set of universal primers were used in pure yeast cultures, PCR sensitivity for Saccharomyces exiguus was 2.5 pg (approx. 10 2 cells), for Candida mogii 12 pg (approx. 10 3 cells), and for Saccharomyces cerevisiae 20 pg (approx. 10 3 cells). In mixed yeast cell cultures, there was a 1000-fold (approx. 10 5 ～ 10 6 cells) decrease in sensitivity under the same PCR conditions.