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藥物食品分析 MEDLINESCIEScopus

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篇名 市售柚類果實、果汁、及柚糖中柚皮苷之含量分析
卷期 6:3
並列篇名 Determination of Naringin in Marketed Fruits, Juices and Candy from Shaddock
作者 徐素蘭黃堂彥侯鈺琪曹智菀李珮端
頁次 599-604
關鍵字 柚皮苷柚類高效液相層析法NaringinShaddockHigh performance liquid chromatographyHPLCMEDLINEScopusSCIE
出刊日期 199809

中文摘要

本研究以省產文旦柚與自柚的外果皮、中果皮、果肉、市售柚類果汁及柚糖為材料,利用高效液相層析法定量其柚皮苷成分,以Hypersil ODS (5μm,250X4.6mm)管柱為固定相,水和乙腈(81:19)之混合液為移動相,流速0.8ml/min,檢測波長為280nm,6,7-dimethoxycoumarin為內標準品。
確效試驗顯示,此方法之精密度尚佳(變異係數為0.3~5.0 %) ,回收率良好(76.9~102.2 %)。定量結果發現文旦柚及白柚所含之柚皮苷成分,以中果皮為最多(分別為23.4 mg/g及16.8mg/g),外果皮次之,果肉最少。另外柚類果汁、柚糖僅含微量柚皮苷。

英文摘要

High performance liquid chromatography was used to determine the contents of naringin in the exocarp, mesocarp and pulp of shaddock and white shaddock sold in markets. The analysis was carried out on an Hypersil ODS column (5 μm, 250 x4.6 mm), using water-ace-tonitrile (81:19) as mobile phase at a flow rate of 0.8ml/min. The detection was set at 280 nm and 6,7-dimethoxycoumarin was used as the internal standard.
The precision of this method (C.V. 0.3~5.0%) and the recoveries (76.9~ 102.2%) were satisfactory for various shaddock fruits and juices. The results indicated the contents of naringin in mesocarp were the highest for both shaddock and white shaddock(23.4 mg/g and 16.8mg/g, respectively), whereas exocarp and pulp contained a lesser amount.0nly a small amount of naringin was found in shaddock juices and shaddock candy.

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