本研究發展出以高效液相層析法檢驗作物中亞喜芬(acifluorfen)及本達隆(bentazone)殘留量之方法，並進行市售乾豆類及米類計二十件農產品之抽樣調查。亞喜芬及本達隆屬弱酸性殺草劑，在鹼性下會解離而溶於水中，添加鹼性對偶離子試劑後形成複合物而溶於二氯甲皖中，本方法即利用此一選擇性對偶離子萃取技術來淨化檢體。亞喜芬以乙?穭峇A?禲G水(9:1,v/v)自作物中抽出，本達隆以丙酮抽出，減壓濃縮至無溶媒後以pH8.0磷酸緩衝液溶出，以正已烷及二氯甲烷去雜質，添加鹼性對偶離子試劑後以二氯甲烷萃取，所得檢液以高效液相層析儀配合C?睄h析管及UV檢測器進行分析，亞喜芬於283nm波長，本達隆於254nm波長偵測。添加亞喜芬於紅豆中，檢體濃度為0.25～0.15ppm，所得平均回收率為91.6～97.6%，最低檢出限量為0.02ppm；添加本達隆於白米及紅豆中，檢體濃度為0.25～0.75ppm，所得平均回收率為88.6～91.7%，最低檢出限量為0.05ppm。以建立之檢驗方法分析市售檢體，乾豆類十件並未檢出亞喜芬，而乾豆類及米類計十件亦未檢出本達隆。

　　　　　Methods using high performance liquid chromatography (HPLC) were developedto determine acifluorfen and bentazone in crops. Twenty samples of dry bean cropsand rice were purchased from traditional markets and analyzed. Acifluorfen andbentazone being weak acids, were selectively extracted as ion pair compounds formedwith tetrabutylammonium ion into dichloromethane under basic conditions. Thistechnique was used for cleanup prior to determination of acifluorfen and bentazoneby reverse phase liquid chromatography with UV detector. A residue of acifluorfenwas extracted from crops with acetonitrile and acetonitrile-H?烙 (9:1, v/v) and aresidue of bentazone was extracted from crops with acetone. The extracts wereevaporated to dryness, dissolved in pH 8.0 phosphate buffer, and washed with n-hexane and dichloromethane. Ion pair compounds were formed by addingtetrabutylammonium ion into an aqueous phase, and then extracted intodichloromethane. The dichloromethane phase was evaporated and the residues weredissolved in organic solvent and determined by HPLC. Recovery studies were carriedout by spiking the standards of acifluorfen and bentazone at the levels of 0.05-0.15 and 0.25-0.75 ppm, respectively. The average recoveries were determined to be91.6-97.6% and 88.6-91.7%, respectively. The detection limits in crops were0.02 and 0.05 ppm for acifluorfen and bentazone, respectively. No residues ofacifluorfen or bentazone were detected in dry bean crop samples, nor was bentazoneresidue found in rice samples.